ASTM D7789-12(R2021) pdf free download

ASTM D7789-12(R2021) pdf free download.Standard Practice for Collection of Fungal Material from Surfaces by Swab
1. Scope
1.1 The purpose of this practice is to describe the proce- dures for collection of surface samples using sterile swabs. 1.2 The purpose of this practice is to support the field investigator in differentiating fungal materials from non-fungal material such as scuffs, soot deposits, stains, pigments, dust, efflorescence, adhesives, dust, and water stains. 1.3 This practice does not address building occupant exposures, or occupant health risks. 1.4 The samples collected by this practice are appropriate for culture, direct microscopy, and biochemical analysis, or combination thereof. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appro- priate safety, health, and environmental practices and deter- mine the applicability ofregulatory limitations prior to use. 1.6 This international standard was developed in accor- dance with internationally recognized principles on standard- ization established in the Decision on Principles for the Development of International Standards, Guides and Recom- mendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
3. Terminology
3.1 Definitions: 3.1.1 sample, n—a portion of a population intended to be representative ofthe whole. a portion ofa population. Aportion of material that is taken for testing or record purposes. D6044 3.1.2 sampling, n—a process consisting ofthe withdrawal or isolation of a fractional part of the whole. D1356 3.2 Definitions ofTerms Specific to This Standard: 3.2.1 area (surface, sampled), n—a defined, measured ex- tent of surface sampled. 3.2.2 biochemical analysis, n—act of assessing chemical substances (that is, chitin, enzyme activity, ergosterol, mycotoxin, DNA) originating from living or dead organisms. 3.2.3 chain ofcustody (COC), n—a document that provides for the traceable transfer of field samples to the analytical laboratory. It may or may not be combined with the field data sheet. 3.2.4 culture analysis, n—act of assessing microorganisms (if present) capable of growing on selected media under artificial and controlled conditions. 3.2.5 direct microscopy analysis, n—act of assessing micro- organisms (if present) using an optical compound microscope. 3.2.6 field data sheet, n—a record of varying names that provides a reference document for information directly related to the sample collection event, including pre- and post- calibration data. 3.2.7 fungal material, n—fungal spores, hyphae and repro- ductive structures. 3.2.8 fungi, n—eukaryotic, heterotrophic, absorptive organ- isms that develop a rather diffuse, branched, tubular body (that is, network ofhyphae) and reproduce by means ofspores. 3 The terms ‘mold’ and ‘mildew’ are frequently used by laypersons when referring to various fungal colonization. For purposes of this practice, these terms have no technical meaning.
4. Significance and Use
4.1 This practice provides a procedure for collecting surface material using a sterile swab. 4.2 A swab sample collected according to this practice is intended to be used to assess fungal material on surfaces.4.3 A swab sample collected from an area with defined dimensions can be used to quantify fungal material per unit area or for qualitative analysis. 4.4 A swab sample collected from point(s) ofinterest can be used for qualitative analysis or to quantify fungal material per sample. 4.5 A swab sample collected according to this practice can be analyzed by direct microscopy, culture, or biochemical analysis.
5. Materials and Supplies
5.1 Swabs: 5.1.1 Must be sterile and have a sterile transport container. 5.1.2 May be used dry or wetted. 5.1.2.1 Wetting/transport media (not required for dry swabs) must be appropriate for the intended analysis. 5.2 Ruler, template, or equivalent for measuring sample area. 5.3 Permanent marking pen or labels for sample identifica- tion. 5.4 Chain ofcustody (COC). 5.5 Field data sheet, if separate from the COC. 5.6 Temperature controlled transportation container, as ap- propriate.
6. Procedure
6.1 Document sample location/surface. 6.2 Document the dimensions of the sampled area, if appropriate. 6.3 Select appropriate swab type (dry or wetted) to achieve optimum collection efficiency. 6.4 Remove swab from container and maintain sterility by taking care not to contact any surface, except the area to be sampled. 6.4.1 For a dimensional area sample, rub swab across sampling area in a back and forth motion, both horizontally and vertically, slightly turning the swab with each pass until entire area is sampled and the maximum material has been collected from the surface. 6.4.2 For a point of interest sample, touch the swab to the surface. 6.5 Return swab to sterile container for transport. 6.6 Label the sample container with a unique identifier. 6.7 Complete field data sheet in a physical or electronic format.